Skip to main content
. 2017 Mar 29;8(22):36137–36148. doi: 10.18632/oncotarget.16661

Figure 1. MACS purification of GSCs from U-251 cells and characterization of GSCs by flow cytometry.

Figure 1

GSCs were defined as CD133+ cells, which were separated by using MACS and assessed by flow cytometry for determining the efficiency of purification. (A) representative flow plots showing the percentage of CD133+ cells in U-251 cells and the purified GSCs; left curve: isotype control; right curve: antibody; (B) summarized data showing the percentage of CD133+ cells before and after MACS; *p < 0.05, vs. U-251 cells. Data are expressed as mean ± SEM; n = 3/group. MACS: magnetic activated cell sorting.