Table 1. Somatic mutations in the components of MAP kinase, Wnt/β-catenin and NF-κB Signaling pathways in GBCs.

Genes	Mutation types	Allele change	Amino acid change	Case No.	Pathways
ADAM12	Nonsynonymous	Exon16: c.1619-7G > C	Splicing	T7	MAP kinase
KRAS	Nonsynonymous	Exon3: c.183A > T	p.Q61H	T16	MAP kinase
MAPKBP1	Nonsynonymous	Exon30: c.3722C > T	p.P1241L	T9	MAP kinase
	Nonsynonymous	Exon29: c.3704C > T	p.P1235L	T9	MAP kinase
MAX	Nonsynonymous	Exon3: c.202A > G	p.K68E	T36	MAP kinase
	Nonsynonymous	Exon4: c.229A > G	p.K77E	T36	MAP kinase
NF1	Nonsynonymous	Exon14: c.1641+1G >A	Splicing	T4,16,17	MAP kinase
	Nonsynonymous	Exon52: c.7676-2A>T	Splicing	T4,16,17	MAP kinase
	Nonsynonymous	Exon53: c.7739-2A>T	Splicing	T4,16,17	MAP kinase
	Nonsynonymous	Exon15: c.1645C > G	p.L549V	T4,16,17	MAP kinase
PDGFRA	Nonsynonymous	Exon17: c.2381A > G	p.D794G	T7	MAP kinase
RRAD	Nonsynonymous	Exon4: c.371-8C > T	Splicing	T30	MAP kinase
APC	Frameshift deletion	Exon16: c.4385_4388del	p.1462_1463del	T14	Wnt/β-catenin
	Frameshift deletion	Exon14: c.4331_4334del	p.1444_1445del	T14	Wnt/β-catenin
	Frameshift deletion	Exon17: c.4385_4388del	p.1462_1463del	T14	Wnt/β-catenin
CCND1	Nonsynonymous	Exon4: c.718G > A	p.D240N	T9	Wnt/β-catenin
CTNNA1	Stopgain SNV	Exon3: c.265G > T	p.E89X	T9	Wnt/β-catenin
CTNNB1	Nonsynonymous	Exon3: c.134C > T	p.S45F	T16,17	Wnt/β-catenin
CARD10	Nonsynonymous	Exon20: c.3077G > A	p.C1026Y	T7	NF-κB
CARD11	Nonsynonymous	Exon25: c.3260+3A>G	Splicing	T9	NF-κB
IRAK2	Nonsynonymous	Exon12: c.1511G > A	p.R504Q	T9	NF-κB
PDCD11	Nonsynonymous	Exon18: c.2524G > A	p.E842K	T16	NF-κB
RELA	Stopgain SNV	Exon3: c.115G > T	p.E39X	T14	NF-κB