Table 3. Primer sequences used to amplify bisulphite converted DNA in qMSP analysis.
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Product (bp) | Tm(°C) |
|---|---|---|---|---|
| SFRP1 | GAAGAGCGAGTAGAGGAA | ACACGAAACCATAACGAAA | 103 | 58 |
| SFRP2 | AAGAGCGAGTATAGGAAT | CCTACCAACCTACAACTA | 167 | 56 |
| WIF1 | TCGGAGAAGGGTATTTAGAGA | CCATCATCAACACTCAATCAA | 123 | 58 |
| PRKCB | TGTAAGTGTGTGCGGTTAT | CCCATCCATCCCATTAATCA | 96 | 58 |
| ACTB(1) | GTGATGGAGGAGGTTTAGTAAGTT | CCAATAAAACCTACTCCTCCCTTAA | 129 | 56 |
| ACTB(2) | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAA | 133 | 58 |
The primers of ACTB (1) were designed for 56 annealing temperature. The primers of ACTB (2) were designed for 58 annealing temperature.