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. 2017 Mar 31;8(22):36368–36382. doi: 10.18632/oncotarget.16755

Figure 5. Activation of ERα by 17β-estradiol significantly induced miRNA production that bound to the 3′UTR of the p21 mRNA construct.

Figure 5

T47D (A) and PC3 cells (B) were transfected with the luciferase-p21 3′UTR plasmid followed by 1 nM 17β-estradiol treatment. Luciferase activity was then measured after 72 hours. Both T47D (C) and PC3 (D) cells were transfected with the luciferase-p21 3′UTR plasmid and treated with DMSO control (left panel) or 10 nM ICI-182780 (right panel) in the presence of 1 nM 17β-estradiol for 20 hours. Luciferase activity was then measured. Each luciferase activity value from vehicle-treated cells was set as 100. Data are presented as mean ± S.D.; *P < 0.05 and **P < 0.01 (compared with vehicle treatment).