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(A) Western blotting was performed to determine the expression of PI3K and AKT. (B) qReal-time PCR was performed with PIK3R1 (p85-a), PIK3R2 (p85-b); regulatory subunits, primer as PI3CA (p110-a; catalytic) subunits. MAPK family assessed by immunoblot for ERK, JNK and p38 (C).
