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. 2017 Mar 28;8(22):36553–36565. doi: 10.18632/oncotarget.16615

Figure 2. Detection of the expression of the identified miRNAs at different developmental stages.

Figure 2

(A) The expression levels of FSHb were determined via quantitative RT-PCR with GAPDH as an internal standard in the pituitary cells of 15-day-old and 4-month-old rats. (B) The expression levels of some of the identified miRNAs were determined through quantitative RT-PCR with U6 as an internal standard at the different developmental stages of the rat pituitary. (C-D) In rat anterior pituitary cells from different developmental stages, derived from 15-day-old and 4-month-old rats, the expression levels of miR-21-3p and miR-433 were separately determined via quantitative RT-PCR with U6 as an internal standard. The data are presented as the mean ± standard deviation from at least three independent experiments; statistical significance was determined using multiple comparisons; and P<0.05 was considered significant. The panels with different letters were considered statistically significant (P<0.05).