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. 2017 Apr 13;116(10):1340–1349. doi: 10.1038/bjc.2017.100

Figure 6.

Figure 6

Influence of HIF-1α on ROS production in FaDu-CSCs and SQ20B-CSCs. Both CSC cell lines were transfected with an irrelevant siRNA or a siRNA targeting HIF-1α and the ROS production was measured with the CM-H2DCFDA dye and analysed by FACS under hypoxia or after 10 Gy photons in normoxic or hypoxic conditions from 30 min to 24 h. Values represent the mean±s.d. Globally, no differences were observed between cells transfected with the siRNA against HIF-1α and the irrelevant siRNA. *P<0.05; **P<0.01 and ***P<0.005.