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. Author manuscript; available in PMC: 2018 Jun 22.
Published in final edited form as: Cell Chem Biol. 2017 May 4;24(6):673–684.e4. doi: 10.1016/j.chembiol.2017.04.009

Figure 6. ACSF3 Does Not Affect Mitochondrial Protein Acetylation or Succinylation.

Figure 6

(A) Immunoblotting for acetylated lysine residues in whole cell and mitochondrial extracts of cells in the presence or absence of 5 mM malonate for 24h. Total protein staining by amido black shown as loading control.

(B-C) Immunoblotting for malonylated (B) and succinylated (C) lysine residues in mitochondrial extracts of ACSF3 KO and control cells treated with 5 mM malonate, 5 mM 3-nitropropanoate (3-NP, SDH suicide inhibitor), or both for 24h. See also Figure S5.