| Antibodies |
| Acyl-CoA synthetase family member 3 (ACSF3), rabbit polyclonal |
ThermoFisher |
Cat#PA5-25803 |
| Heat shock chaperone 70 (HSC70), mouse monoclonal |
Santa Cruz |
Cat#sc-7298 |
| MitoProfile total OXPHOS, mouse monoclonal antibody mix |
Abcam |
Cat#ab110413 |
| Voltage-dependent anion channel 1 (VDAC1), rabbit polyclonal |
Calbiochem |
Cat#AB10527 |
| Aconitase 2 (ACO2), rabbit polyclonal |
Cell Signaling |
Cat#6922 |
| α-lipoic acid, rabbit polyclonal |
Calbiochem |
Cat#437695 |
| Phospho-acetyl-CoA carboxylase (ACC) (Ser79), rabbit polyclonal |
Cell Signaling |
Cat#11818 |
| Total ACC, rabbit polyclonal |
Cell Signaling |
Cat#3676 |
| α-malonyl-lysine, rabbit monoclonal antibody mix |
Cell Signaling |
Cat#14942 |
| α-acetyl-lysine, rabbit polyclonal |
Cell Signaling |
Cat#9441 |
| α-succinyl-lysine, rabbit polyclonal |
PTM Biolabs |
Cat#401 |
| GFP, mouse monoclonal |
Santa Cruz |
Cat#sc-9996 |
| Cy3-conjugated anti-mouse secondary antibody |
Invitrogen |
Cat#M30010
|
| Horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody |
GE Healthcare |
Cat#NA931V |
| HRP-conjugated anti-rabbit secondary antibody |
GE Healthcare |
Cat#NA934V |
| GFP nanobody magnetic beads |
Allele Biotechnology |
Cat#ABP-NAB-GFPM050 |
| Chemicals, Peptides, and Recombinant Proteins |
| Malonate |
Sigma-Aldrich |
M1296; CAS: 141-82-2 |
| [U-14C]D-glucose |
Moravek Biochemicals |
MC144W |
| [U-14C]L-glutamine |
New England Nuclear/PerkinElmer |
NEC451050UC |
| [U-14C]L-alanine |
Moravek Biochemicals |
MC132 |
| [3H]acetate |
New England Nuclear/PerkinElmer |
NET003025MC |
| [2-14C]malonate |
Moravek Biochemicals |
MC312 |
| [1,3-13C]malonate |
Sigma-Aldrich |
490199; CAS: 99524-14-8 |
| 3-Nitropropionic acid |
Sigma-Aldrich |
N5636; CAS: 504-88-1 |
| Critical Commercial Assays |
|
|
| CellTiter 96 Aqueous One Cell Proliferation Assay |
Promega |
Cat#G3582 |
| Amersham Prime Enhanced Chemiluminescent Substrate |
GE Healthcare |
Cat#RPN2232 |
| XFe96 Extracellular Flux Assay Kit |
Seahorse Bioscience |
Cat#102601-100 |
| CyQUANT Cell Proliferation Assay Kit |
Invitrogen |
Cat#C7026 |
| Complex II Enzyme Activity Microplate Assay |
Abcam |
Cat#ab109908 |
| Experimental Models: Cell Lines |
| Human: HEK293T |
ATCC |
Cat#CRL-3216 |
| Experimental Models: Organisms/Strains |
| Mouse: Wild-type C57BL/6J |
The Jackson Laboratory |
JAX: 000664 |
| Mouse: ob/ob obese, leptin-deficient: B6.Cg-Lepob/J |
The Jackson Laboratory |
JAX: 000632 |
| Oligonucleotides |
|
|
| ACSF3 Forward: 5′-ACACAGAGGAAGTGGTCTTCT-3′ |
This paper |
N/A |
| ACSF3 Reverse: 5′-CGTTAGCGCATAGGAAGGAGA-3′ |
This paper |
N/A |
| Recombinant DNA |
|
|
| Human codon-optimized Cas9 expression vector |
Mali et al., 2013 |
Addgene Plasmid #41815 |
| pEF6-nls-YFP-2A-Cas9 for bicistronic expression of nuclear YFP and Cas9 |
Bowman et al., 2016 |
N/A |
| Mitochondrially-targeted YFP expression vector (pEYFP-Mito) |
Clontech |
Cat#6115 |
| Cytoplasmic YFP expression vector (pEYFP-C1) |
Clontech |
Cat#6341 |
| Other |
| FuGENE HD Transfection Reagent |
Promega |
Cat#E2311 |
| In-Fusion HD Cloning Enzyme |
Clontech |
Cat#639636 |
