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. Author manuscript; available in PMC: 2018 Jun 23.
Published in final edited form as: Circ Res. 2017 Apr 12;121(1):56–70. doi: 10.1161/CIRCRESAHA.117.310870

Figure 7. MPO-dependent fibroblast transdifferentiation.

Figure 7

(A) Representative immunofluorescence stainings for fibroblast marker DDR2 (green) and myofibroblast marker α-SMA (red) within the infarct area of WT and Mpo-/- mice after left ventricular ischemia and 7 days of reperfusion (I/R) and 5 days of permanent ischemia (PI) (blue=DAPI; scale bar=200 μm). (B) Quantitative analysis of myofibroblasts within the infarct and periinfarct region after 7 days of I/R and 5 days PI; WT / Mpo-/- 7d I/R n=11/15, WT / Mpo-/- 5d PI n=5/5. (C) Relative α-SMA protein expression of isolated cardiac fibroblasts after 8 hours of MPO and PDGF treatment. (D) Relative collagen I expression of isolated cardiac fibroblasts after 36 hours of MPO treatment. (E) Relative α-SMA protein expression of isolated cardiac fibroblasts after co-culture with WT or Mpo-/- leukocytes. (F) Relative phosphorylation of p38 MAP-kinase (p-p38/p38 MAPK) in isolated fibroblasts upon 15 minutes of MPO treatment. Original immunoblots are shown in Online Figure IX-XII. Mean ± SEM is shown. C, D, E, F: n=4 independent experiments. *=P<0.05, **=P<0.01, ***=P<0.001, by unpaired Students's t-test for B, D, E and by Kruskal Wallis test followed by Bonferroni post hoc test for C, F.