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. 2017 Jun 23;7:4139. doi: 10.1038/s41598-017-04064-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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YvcK regulates cell length. (A) Growth curve of WT strain (168) and ΔyvcK mutant strain (SG471) on minimal medium with sorbitol as sole carbon source. (B) Histogram of cell lengths for 168 and SG471 strains collected at OD₆₀₀ = 0.4: WT (n = 306), ΔyvcK strain (n = 335). (C) Histogram of cell lengths for WT strain (168), ΔyvcK strain (SG471) and ΔyvcK strain with P _xyl yvcK at amyE locus (SG517). These three strains were grown in LB medium in the presence of xylose and collected at OD₆₀₀ = 0.4: WT (n = 319), ΔyvcK strain (n = 256), ΔyvcK, P _xyl yvcK strain (n = 301). (D) Analysis of YvcK production from crude extracts of WT strain (168), ΔyvcK strain (SG471) and ΔyvcK strain with P _xyl yvcK at amyE locus (SG517) by Western blotting. Bacteria were collected at OD₆₀₀ = 0.4, lyzed and 10 μl of cell extracts were run on a 12.5% SDS-PAGE and transferred to nitrocellulose membrane by electroblotting. The blot membrane was exposed for each time set in interval time and accumulates images. Full-length blot is presented in Supplementary data.