Figure 2.

AngII infusion promotes regulatory B cells in B cell replenished Apoe ^−/− /Baffr ^−/−mice. (a) Representative dot plots and quantitative analysis of flow cytometry staining of splenic B cell populations. Marginal zone (MZ) B cells are defined as CD21^hiCD23⁻ population and follicular B cells (FO) are defined as CD21⁺CD23⁺ population amongst B220⁺ cells. Adoptive transfer of Apoe ^−/− /Baffr ^−/− mice with B cells without (left) or with (right) AngII demonstrates replenishment of all B cell subsets. n = 6–8 mice per group. ***p < 0.001. (b) Representative dot plots and quantitative analysis of facs staining of splenic regulatory CD1d^hiCD5⁺ B cells gated on B220⁺ cells in Apoe ^−/− /Baffr ^−/− mice with or without adoptive B cell transfer, infused with PBS for 28 days (left) and their age matched controls with AngII infusion (right). (c) Representative FACS examples and quantitative analysis of splenic CD44^hi cells amongst CD4⁺ T cells in Apoe ^−/− /Baffr ^−/− mice with or without adoptive B cell transfer, infused with PBS for 28 days (left) and their age matched controls with AngII infusion (right). (d) Representative examples and quantitative analysis of splenic IFN-γ⁺ cells amongst CD4⁺ T cells by intracellular facs staining in Apoe ^−/− /Baffr ^−/− mice with or without adoptive B cell transfer, infused with PBS for 28 days (left) and their age matched controls with AngII infusion (right). n = 6–8 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, ns denotes non significance.