Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 23;7:4165. doi: 10.1038/s41598-017-04220-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Embryos and their nuclear architecture visualized by OCM. (a) Stages in oogenesis and embryonic development subjected to OCM scanning. At some stages only intracellular structures were visualized (structural imaging in a single time-point), in other – both the structures and the dynamics of cellular processes such as pronuclei formation and movement or cell divisions were imaged (time-lapse imaging). (b–e) Pronuclei and nucleoli in a mouse zygote (b), 2-cell embryo (c) and blastocyst (d), and in a porcine 1-cell embryo obtained by parthenogenetic activation of an oocyte (e). BF – bright field images. OCM images of mouse zygotes and 2-cell embryos (DTIsp #3 protocol) were processed with the minimum intensity projection over 15 μm (Procedure #2). The mouse blastocyst was imaged using DTIsp #3 protocol without spatial averaging (Procedure #1). OCM images of a porcine embryo (DTIsp #2) are shown without spatial averaging (Procedure #1). Asterisks indicate the nucleoli, arrows - specular light reflexes visible in the nuclei that label the nucleoli. Cropped regions are encircled with a dashed line. Scale bars represent 20 μm. See also Supplementary Videos S1–S3 and S14 for more images of these cells.