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. 2017 Jun 23;7:4122. doi: 10.1038/s41598-017-04547-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytokine production in the skin after EP. The cytokine concentrations were measured in the supernatants of skin biopsies after 6 h of incubation at 37 °C and 5% CO₂. The values of the change fold were expressed as the ratio between the cytokine concentrations in the supernatant in each condition and the ones measured in the non-electroporated condition. Skin biopsies were performed 24, 48 and 72 hours after intradermal injection of PBS followed by EP. Kruskal-Wallis test (n = 5). Data are presented as mean ± SD; *p < 0.05, EP: electroporation.