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. 2017 Jun 23;7:4150. doi: 10.1038/s41598-017-04386-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasma exosomes and their RNA sizes. (A) Scanning electron microscopy revealed the phenotype of the exosomes isolated from plasma. Exosomes isolated with ExoQuick were fixed and processed with STM. The bar represents 100 nm. (B) Exosomal RNAs determined by small RNA chip. A total of 50 μL exosome solution from 250 μL plasma was subjected to RNA extraction. The column binding RNA was resolved in 14 nuclease free water. One microliter of the RNA solution was added in the small RNA chip and processed with Bioanalyzer 2100. Shown is the representative gel electrophoresis map.