Table 1.
Comparison of protein expression between magainin I-susceptible and magainin I-resistant Escherichia coli strains identified via nanoUPLC-MSE and their participation in the bacterial biological process.
| Accession Number | UniProt Identification | Protein Expression | Score | Log (e) Ratio* | Variance* | Protein Description | Molecular Mass (Da) | Biological Classes |
|---|---|---|---|---|---|---|---|---|
| P0A915 | OMPW_ECOLI | Upregulated | 236.44 | 0.50 | 0.45 | Outer membrane protein W | 2292.8 | Environmental Information Processing |
| P06996 | OMPC_ECOLI | Upregulated | 3576.12 | 0.65 | 0.03 | Outer membrane protein C | 4036.8 | Environmental Information Processing |
| P02931 | OMPF_ECOLI | Upregulated | 639.19 | 0.78 | 0.07 | Outer membrane protein F | 3933.3 | Environmental Information Processing |
| Q1PI90 | Q1PI90_ECOLI | Upregulated | 2207.53 | 0.71 | 0.04 | Outer membrane protein 1b (Fragment) | 2759.4 | Environmental Information Processing |
| P75678 | YKFA_ECOLI | Upregulated | 283.62 | 0.52 | 0.51 | Uncharacterized protein | 3189.2 | Uncharacterized protein |
| P23843 | OPPA_ECOLI | Upregulated | 558.97 | 0.67 | 0.05 | Periplasmic oligopeptide-binding protein | 6089.9 | Environmental Information Processing |
| P39165 | YCHO_ECOLI | Upregulated | 387.49 | 0.68 | 0.33 | Uncharacterized protein | 5210.0 | Uncharacterized protein |
| P0AAA9 | ZRAP_ECOLI | Upregulated | 14392.98 | 0.70 | 0.05 | Zinc resistance-associated protein | 1519.9 | Environmental Information Processing |
| P03841 | MALM_ECOLI | Upregulated | 311.79 | 0.74 | 0.22 | Maltose operon periplasmic protein | 3194.3 | Environmental Information Processing |
| P0ADE6 | YGAU_ECOLI | Upregulated | 772.28 | 0.80 | 0.13 | Uncharacterized protein | 1606.3 | Uncharacterized protein |
| P13033 | GLPB_ECOLI | Downregulated | 483.13 | 2.03 | 0.36 | Anaerobic glycerol-3-phosphate dehydrogenase subunit B | 4535.7 | Metabolism |
| P09394 | GLPQ_ECOLI | Downregulated | 2564.38 | 1.40 | 0.18 | Glycerophosphoryl diester phosphodiesterase | 4084.3 | Metabolism |
| P0A9C0 | GLPA_ECOLI | Downregulated | 2024.03 | 1.28 | 0.18 | Anaerobic glycerol-3-phosphate dehydrogenase subunit A | 5895.8 | Metabolism |
| P0AB14 | YCCJ_ECOLI | Downregulated | 42340.24 | 0.80 | 0.07 | Uncharacterized protein | 8524.0 | Uncharacterized protein |
| P76268 | KDGR_ECOLI | Downregulated | 403.70 | 0.79 | 0.30 | Transcriptional regulator kdgR | 3002.9 | Genetic Information Processing |
| P64463 | YDFZ_ECOLI | Downregulated | 12233.5 | 0.77 | 0.05 | Putative selenoprotein ydfZ | 7276.0 | Metabolism |
| P77454 | GLSA1_ECOLI | Downregulated | 485.06 | 0.74 | 0.17 | Glutaminase 1 | 3290.3 | Metabolism |
| P0AAS7 | YBCJ_ECOLI | Downregulated | 854.17 | 0.60 | 0.37 | Uncharacterized protein | 7390.0 | Uncharacterized protein |
| P0AGL2 | TDCF_ECOLI | Downregulated | 826.76 | 0.57 | 0.16 | Protein tdcF | 1400.7 | Metabolism |
| Q59385 | COPA_ECOLI | Downregulated | 205.89 | 0.53 | 0.36 | Copper-exporting P-type ATPase A | 8787.3 | Environmental Information Processing |
| P0A6K3 | DEF_ECOLI | Downregulated | 392.11 | 0.52 | 0.46 | Peptide deformylase | 1932.8 | Metabolism |
| P0A759 | NAGB_ECOLI | Downregulated | 276.4 | 0.51 | 0.37 | Glucosamine-6-phosphate deaminase | 2977.4 | Metabolism |
| P0ABT2 | DPS_ECOLI | Downregulated | 32750.34 | 0.51 | 0.03 | DNA protection during starvation protein | 1869.5 | Genetic Information Processing |
| P0A6Y8 | DNAK_ECOLI | Downregulated | 19211.09 | 0.51 | 0.02 | Chaperone protein DnaK | 6911.5 | Genetic Information Processing and Metabolism |
| P0A8G6 | WRBA_ECOLI | Downregulated | 11264.48 | 0.50 | 0.03 | Flavoprotein wrbA | 2084.6 | Genetic Information Processing and Metabolism |
*Protein differential expression was considered significant in a Log(e) ratio ≥0.5 and a variance value ≤0.51.