Figure 8.

Leptin replacement did not affect pancreatic β cell mass and proliferation in OSI-906-treated mice. (a) Pancreatic sections were stained with antibodies to insulin. The scale bar represents 300 μm. β Cell mass with β cell area shown as the percent area of the entire pancreas. Data represent the mean ± SEM. *P ≤ 0.05 (n = 6 per group). (b) Pancreatic sections were stained with antibodies to DAPI (blue), insulin (green), and BrdU (red). Scale bar = 50 μm. Number of BrdU-positive β cells in the islets (at least 50 islets per indicated group). Data represent the mean ± SEM. *P ≤ 0.05, **P ≤ 0.01 (n = 6 per group).