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. 2017 Jun 23;7:4149. doi: 10.1038/s41598-017-03510-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electron microscopy detects close proximity of NCC and ENaC in murine kidney cortex after aldosterone treatment. Immunogold labeling of NCC (12 nm particles) and γENaC (6 nm particles) in mouse DCT2 of (A,B) vehicle (25% DMSO in saline) and (C–J) Aldo-treated kidney cortex. The inserts (A–D) show a magnified view of closely associated NCC and γENaC. Figures E–J are magnified views of increased NCC- γENaC intracellular colocalization, close to the cell surface (E–G) and in intracellular vesicles (H–J). Scale bar = 1 μm.