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. 2017 Jun 23;7:4149. doi: 10.1038/s41598-017-03510-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coimmunoprecipitation demonstrated increased association of NCC and αENaC aldosterone-treated mice. (a) Mouse kidney cortex homogenates were used and CoIP performed; CoIP fraction probed with αENaC antibodies in vehicle (25% DMSO in saline) or Aldo-treated (4 μg) mice for 10 days, fed high salt (4%) chow. (b) Whole kidney cortex lysate probed with αENaC. (c) Representative blots for CoIP experiments. Blots were cut to assay for multiple antibodies. (d) Whole kidney cortex homogenate probed with NCC and (e) representative blots for NCC (lysate and and CoIP fraction) and loading control (GAPDH). Blots were cut to assay for multiple antibodies. Separation in images shown with a space. Data represented as mean ± SEM, n = 6; Wilcoxin Signed-Rank, *p < 0.05.