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. 2017 Jun 15;144(12):2294–2305. doi: 10.1242/dev.146019

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Sox9 is expressed throughout the development of the submandibular gland. (A-F) Sox9 immunofluorescence (red) at the placode [E11.0 (A), E11.5 (B)], initial bud [E12.5 (C)], pseudoglandular [E13.5 (D)], canalicular [E15.5 (E)] and adult (F) stages. DNA is shown in blue (DAPI), F-actin in yellow and Mist1 in green. Dotted lines in A-D delineate the salivary gland epithelium. Insets in F show magnifications of an acinus stained for Mist1 (green) and Sox9 (red). Arrowheads point to Sox9-positive cells within the striated duct. G, ganglion; MC, Meckel's cartilage; SL, sublingual gland; SMG, submandibular gland. Scale bars: 100 μm.