Fig. 4.
Antioxidant gene expression decreases and ROS levels increase in Kat8Gdf9 cKO oocytes. (A) Relative mRNA levels of the antioxidant genes Prdx1, Prdx2, Gpx1 and Gpx4 in oocytes from 2-week-old WT and Kat8Gdf9 cKO females as detected by real-time PCR. The mRNA levels in WT oocytes were arbitrarily set to 1, and values were normalized to Actb. (B) Oocytes isolated from 2-week-old mice were treated with 5(6)-carboxy-2′7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) for ROS detection. Images shown are representative of three independent experiments. Scale bar: 100 μm. (C) ROS levels in B were quantified by measuring carboxy-DCF fluorescence intensity. (A,C) Data are mean±s.e.m. from three independent experiments. *P<0.05, ***P<0.001, t-test.