Fig. 6.
Antioxidant treatment decreases ROS levels, DSBs and apoptosis in Kat8Gdf9 cKO oocytes. (A) Oocytes isolated from 3-week-old PBS- or NAC-injected mice were treated with carboxy-H2DCFDA for ROS detection. (B) ROS levels were quantified by measuring carboxy-H2DCFDA fluorescence intensity. (C) DSBs were detected by γ-H2AX staining in oocyte nuclei from 3-week-old PBS- or NAC-treated mice. (D) Proportions of oocytes containing various numbers of γ-H2AX foci. (E) Detection of apoptosis by TUNEL assay in oocytes from 3-week-old PBS- or NAC-treated mice by TUNEL assay. (F) Percentage of TUNEL-positive oocytes in 3-week-old PBS- or NAC-treated mice. Data (B,F) are mean±s.e.m. from three independent experiments. **P<0.01, ***P<0.001, one-way ANOVA (B,F) or Chi-square test (D). Images (A,C,E) are representative of three independent experiments. Scale bars: 100 μm in A; 10 μm in C; 20 μm in E.