Fig. 3.

Endogenous CLR-1 receptor expression in larval and adult muscles. (A) Cas9 engineering showing the tdTomato insertion site within the clr-1 genomic locus. The C. briggsae unc-119 gene was used for screening. Asterisks indicate downstream genes (*, mrps-16; **, F56D1.2). (B) DIC, fluorescence [tetramethylrhodamine isothiocyanate (TRITC)] and merged images focusing on body wall muscles. In the DIC image, the characteristic rhomboid shapes of body wall muscles are hand drawn in yellow (Francis and Waterston, 1985). Arrowheads point down to locations of clr-1::tdTomato expression along the muscle sarcolemma. Scale bars: 10 µm.