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. 2017 Jun 15;144(12):2175–2186. doi: 10.1242/dev.152025

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — SMN-1 promotes Arp2/3 activity in muscle. (A) Muscle mitochondria in control, mutant, and RNAi hermaphrodites. smn-1 reduction of function closely resembles arx-2 reduction of function (Han et al., 2012). The hT2 balancer chromosome has a wild-type copy of smn-1. (B) Merged DIC and fluorescence (TRITC) image showing ARX-2::mCherry localization at muscle myofilament of an smn-1(ok355) mutant. (C) The arx-2::mCherry transgene. (D,E) Muscle mitochondria in nontransgenic (D) and transgenic (E) smn-1(ok355) mutants. Asterisk (A,D,E), nucleus. (F) Body bends per minute of L3 stage worms of the indicated genotypes in liquid medium (N=15). Bars indicate s.d. The wild-type value is 242±21 thrashes per minute. *P<0.01, Student's t-test. Scale bars: 5 µm.