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. 2017 Jun 1;10(6):717–725. doi: 10.1242/dmm.028530

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Adipocyte histomorphometry of epididymal white adipose tissue. (A,B) At study conclusion, area analysis was performed on adipocytes within epididymal white adipose tissue using MetaMorph software. Graph in A shows mean values whereas that in B shows median values. (C-E) Size–frequency distribution was calculated to account for changes in both adipocyte number and size. Six sections, more than 100 µm apart were averaged per mouse. Data are mean±s.e.m. (median±s.e.m. in B) from n=6 mice/group. In A,B, **P<0.01, ***P<0.001, wild-type mice fed HFD versus SD; in C-E, *P≤0.05, wild-type mice fed HFD versus SD; ^#P≤0.05, Lepr^db/+ or Lep^ob/+ mice fed HFD versus SD.