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. 2017 Jun 1;10(6):717–725. doi: 10.1242/dmm.028530

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Measures of nerve fiber function and intra-epidermal nerve fiber density. Sural (A-C) and sciatic (D-F) nerve conduction velocities (NCVs) were measured at 16, 24 and 36 weeks for all groups (n=6/group). Anatomical measures of intra-epidermal nerve fiber density (IENFD) were measured for all groups at 36 weeks of age (G; n=8/group). Data are mean±s.e.m. White circles: wild-type mice fed standard diet (SD); white triangles: db/+ or ob/+ mice fed SD; black circles: wild-type mice fed HFD; black triangles: Lepr^db/+ or Lep^ob/+ mice fed HFD. Ordinary two-way ANOVA with Tukey's multiple comparisons test performed on the four datasets within the individual figures (A-F). Ordinary two-way ANOVA with Bonferroni's multiple comparisons test were performed on IENFD (G). **P<0.01, ***P<0.001, wild-type mice fed HFD versus SD; ^#P<0.05, ^##P<0.01, ^###P<0.001, Lepr^db/+ or Lep^ob/+ mice fed HFD versus SD.