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. 2017 Jun 1;10(6):773–786. doi: 10.1242/dmm.029488

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Fibrosis and inflammation in Umod^+/+, Umod^+/125R and Umod^125R/125R kidneys. (A) Masson's trichrome stain in the corticomedullary region at (i) 8 weeks and (ii) 6 months, and (iii) in the cortex at 6 months, showing fibrous material (blue). (B) Co-immunofluorescent labeling for uromodulin (green) and the T-cell marker CD3 (red) at (i) 8 weeks and (ii) 6 months. Nuclei counterstained with DAPI. (C) Co-immunofluorescent labeling for uromodulin (green) and the macrophage marker F4/80 (red) at (i) 8 weeks and (ii) 6 months. Nuclei are counterstained with DAPI. All scale bars: 50 µm.