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. 2017 Jun 15;6(6):752–764. doi: 10.1242/bio.023200

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — The t^w18 deletion extends over 4.3 Mbp of Chromosome 17. (A) Number of reads obtained by next generation sequencing of t^w18/+ derived genomic DNA mapped to position Chr 17:17000000-25090000. The 3′ end of the deletion is found approximately at position Chr 17:23600000 (asterisk) whereas the 5′ end was roughly positioned between Chr 17: 18.4-19.4 Mb. (B) The presence of DNA elements around the region determined to contain the proximal breakpoint were analyzed by PCR. DNA fragments obtained by PCR amplification and visualized by gel electrophoresis; homozygous t^w18 genomic DNA (lower panel) was amplified up to position Chr 17:19.19 Mb, whereas amplicons were absent from position Chr 17:19.33 Mb, indicating that the deletion starts between position 19.19 and 19.33 Mb. (C) Rough functional assignment of the 74 genes deleted in t^w18.