Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 15;6(6):752–764. doi: 10.1242/bio.023200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 6. — Targeting of the Ppp2r1a locus by homologous recombination. (A) Ppp2r1a targeting construct integrated into the wild-type Ppp2r1a locus (upper panel) by homologous recombination (lower panel). The stop cassette in intron 2 leads to abrogation of transcription. (B) Southern blot analysis of a successfully targeted ESC clone; a BamHI digest reveals a wild-type fragment of 13 kb, the targeted allele shows a 7 kb fragment with the 5′ and a 6.5 kb fragment with the 3′ external Southern probe. (C) PCR genotyping using three primers (P1, P2, P3) distinguishes between wild-type mice and mice carrying the targeted allele. (D) WISH analysis of embryos at E6.5 resulting from Ppp2r1a^+/− inter-crosses shows absence of Ppp2r1a expression in homozygous Ppp2r1a^−/− embryos. Scale bar: 200 µm.