Table 1. List of protein identified by MALDI-TOF/TOF or HPLC-nESI-FTICR in Clostridium septicum extracellular medium.

Banda	Mrb (kDa)	MS methodc	Protein name	SwissProt/NCBInr accession number	Function
1, 17	>200	MALDI	Alpha toxin	Q53482/452163/89257968	Pathogenesis
2	165.3	ESI	Hypothetical protein CPE1875	Q8XJ86/18310857	–
3	128.8	ESI	Pyruvate-flavodoxin oxidoreductase	WP_011969176.1	Oxidoreduction
4	111.1	MALDI/ESI	Collagenase	Q84IM7/28557072	Digestion of collagen
5	83.3	ESI	Formate acetyltransferase	Q8XL89/18310135	Glucose metabolic process
6	83.3	ESI	Formate acetyltransferase	Q8XL89/18310135	Glucose metabolic process
7	60	ESI	60 kDa chaperonin	Q0PVX4/110555138	Chaperone
8	56.7	MALDI/ESI	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	Q0STD7/110803633	Catalyzes interconversion of 2-phosphoglycerate and 3-phosphoglycerate
9	60.3	ESI	Formate-tetrahydrofolate ligase	Q8XHL4/489554783	Tetrahydrofolate interconversion
10	49.7	MALDI/ESI	Alpha toxin Glucose-6-phosphate isomerase	Q53482/452163 Q8XI54/489557801	Pathogenesis; Carbohydrate degradation & glycolysis
11	49.9	MALDI/ESI	Alpha toxin	Q53482/452163	Pathogenesis
	42.0		Flagellin protein FliA(S)	Q8RRA0/19910961	Ciliary or flagellar motility
	41.0		Thiolase	82747493	Acyltransferase
12	35.5	ESI	Hypothetical protein CPE1232	Q8XL10/18310214	–
	38.1		UDP-4-dehydro-6-deoxy-2-acetamido- D-glucose 4-reductase	Q893U5/28211366	Lyase and oxidoreductase activities;
	42.0		Flagellin protein FliA(S)	Q8RRA0/19910961	Ciliary or flagellar motility;
	41.0		Thiolase	82747493	Acyltransferase
13	30.5	MALDI/ESI	3-hydroxybutyryl-coA dehydrogenase	A0Q2T9/118443750	Oxidoreductase
14	30.5	ESI	3-hydroxybutyryl-coA dehydrogenase	A0Q2T9/118443750	Oxidoreductase
15	25.1	ESI	Hypothetical protein CPE1233	Q8XL09/18310215	–
	30.4		Fructose-bisphosphate aldolase	Q97KT9/15894114	Glycolysis
16		MALDI/ESI	Triosephosphate isomerase	Q64G20/52082975	Isomerase
			Hypothetical protein CPE1233	Q8XL09/18310215	–

Notes.

^aBand number as stated in Fig. 1.

^bTheoretical molecular mass (kDa) taken from the UniProtkB entry.

^cType of ionization used for mass spectrometry.