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. 2005 Mar 1;115(3):610–621. doi: 10.1172/JCI23056

Figure 7.

Figure 7

Rescue of ATN by Ngal. (A) Plasma creatinine in mice subjected to 30 minutes of ischemia followed by 24 hours of reperfusion. The first panel shows that holo-Ngal (≥10 μg) from XL1-Blue bacteria (containing siderophore and iron) rescued renal function when introduced 15 minutes before ischemia or within 1 hour after ischemia (+ 1 h). However, Ngal was ineffective when administered later (+ 2 h). The second panel shows that apo-Ngal from BL21 bacteria (siderophore free) was minimally active, but that, when loaded with a siderophore (enterochelin), the protein was protective: both iron-free (apo-Ngal:Sid) and iron-loaded siderophores (apo-Ngal:Sid:Fe) had a protective effect. In comparison, the gallium-loaded complex (apo-Ngal:Sid:gallium) was ineffective, as was a single dose of the iron chelator DFO or the free siderophore (Sid). Retinol-binding protein (RBP), a lipocalin that is also filtered and reabsorbed by the proximal tubule, was ineffective. *P < 0.001 vs. ATN. #P < 0.01 vs. apo-Ngal:Sid (10 μg). The numbers in parentheses show the number of animals analyzed. (B) Preparations of Ngal. Ngal:Sid contains enterochelin, but not iron. Ngal:Sid:Fe contains enterochelin and iron.