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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Virology. 2017 Feb 10;504:122–140. doi: 10.1016/j.virol.2017.01.023

Figure 8. Nuclear co-localization of GPCMV IE2 and guinea pig ND10 components.

Figure 8

gpPML, gpDaxx, gpSp100, and gpATRX were evaluated for its ability to interact with IE2 by cellular co-localization (i) or by GFP trap immunoprecipitation (ii-v). (i) A–D, co-localization of GFP-IE2 (A) and gpPMLmyc (B) shown separately in the same cell. C is the merged image for A and B. D is the overlay of C with DAPI. E–H, GFP-IE2 (E) and gpDaxxFLAG (F). G merged image, H DAPI overlay. I–L, GFP-IE2 (I) and gpSp100HA (J). K merged image, L overlay with DAPI. M–P, GFP-IE2 (M) and gpATRXmyc (N). O merged image, P overlay with DAPI. gpPMLmyc, gpDaxxFLAG, gpSp100HA, and gpATRXmyc detected by primary anti-epitope Ab and secondary anti-mouse or anti-rabbit IgG TRITC. GFP-IE2 detected by fluorescence. (ii) GFP-IE2 and gpPMLmyc co-expression and IP. (iii) GFP-IE2 and gpDaxxFLAG co-expression and IP. (iv) GFP-IE2 and gpSp100HA co-expression and IP. (v) GFP and gpPMLmyc co-expression and IP. GFP-IE2, gpPMLmyc, gpDaxxFLAG, gpSp100HA detected by primary anti-epitope Ab and secondary anti-mouse or anti-rabbit IgG-HRP. Lanes 1 and 4, total cell lysate of transfected GPL cells. Lanes 3 and 6, IP reactions. Lanes 2 and 5, mock transfected GPL cells.