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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Comp Physiol B. 2017 Mar 23;187(5-6):815–825. doi: 10.1007/s00360-017-1081-x

Figure 2.

Figure 2

Platelet fibrinogen binding. Platelets were isolated from non-hibernating squirrels and incubated with Alexa 488 fluorescently labeled fibrinogen. Some samples were activated with different agonists including ADP, TRAP, and PAR4 activating peptide for 5 minutes. Samples were then fixed with paraformaldehyde, analyzed on a flow cytometer, and normalized to matched unstimulated platelet fibrinogen binding. N=5 samples run in triplicate and analyzed by ANOVA, bars with different letters were significantly different, p<0.05.