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Platelets are observed in extrasinusoidal regions of liver during torpor. Frozen sections of liver were prepared from non-hibernating, torpid, and IBA animals. The sections were incubated with rabbit anti-GpIbα and goat anti-rabbit HRP antibodies followed by the chromogenic substrate AEC. Sections were then counterstained with hematoxylin, size bars are 100μm (n=3).
