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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2017 Mar 27;28(7):1324–1332. doi: 10.1007/s13361-017-1600-8

Figure 1.

Figure 1

Comparison of product ion signal from NETD reactions using reagents C16H10•+ and SF5•+ for all precursor charge states of three synthetic peptides (sequences shown top left). The intensities of both a•- and x-type fragment ions were normalized to the total ion current of the unreacted precursor from a preceding scan as explained in the text. Following this normalization, the log2 fold change of the normalized fragment ion current intensity was calculated between SF5•+ and C16H10•+. All precursor ion populations were reacted to the 2τ time point (i.e., 13.5 ± 3% unreacted precursor ion current remaining in the MS/MS spectrum).