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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2017 Mar 27;28(7):1324–1332. doi: 10.1007/s13361-017-1600-8

Figure 3.

Figure 3

Single-scan spectra for the NETD fragmentation of the peptide ETAESYLGAK, z = −2. The unreacted precursor signal accounts for 37.9% and 38.4% of the total ion current in each MS/MS scan for C16H10•+ and SF5•+, respectively, indicating both precursors were reacted to similar extents. Use of SF5•+ as the NETD reagent provided greater sequence coverage than C16H10•+, yielding 11 sequence informative fragment ions compared to 5 for C16H10•+. The NETD reagent SF5•+ produced three CO2 product ion neutral loss species while the fluoranthene spectrum only contains a single CO2 product ion neutral loss fragment. Both spectra were acquired in the nLC-MS/MS experiments and represent a single scan (i.e., un-averaged).