Figure 6.

GC Dynamics across All Immunized Animals

(A–C) GC B cell frequency (A), GC Tfh cell frequency (B), and ratio of GC B cells to GC Tfh cells (C) for all study animals at baseline (BL) and after the first, second, and third immunizations. Points represent individual LNs (n = 144; 72 animals × 2 LNs).

(D–F) Subpopulations of GC Tfh cells. (D) Flow cytometry of GC Tfh cell sub-populations. Gates were set on the basis of total CD4⁺ T cells (Figure S6B). (E) Frequency of FoxP3⁺ GC Tfr cells. (F) Frequency of CXCR3⁺ GC Tfh cells.

(G) GC B cell frequencies separated by gender.

(H) BG505 nAb titers separated by gender.

All nAb titer data represent geometric mean titers with geometric SD. All cell-frequency data represent the mean and SD. See also Figure S6.