REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-Ki67 Alexa Fluor 488 (Clone B56)	BD Biosciences	Cat# 558616; RRID: AB_647087
mouse anti-PD-1 PE (Clone EH12.2H7)	BioLegend	Cat# 329905; RRID: AB_940481
mouse anti-Bcl6 PE-CF594 (Clone K112-91)	BD Biosciences	Cat# 562401; RRID: AB_11152084
mouse anti-ICOS PerCP-cy5.5 (Clone C398.4A)	BioLegend	Cat# 313517; RRID: AB_10639735
mouse anti-CXCR5 PE-Cy7 (Clone MU5UBEE)	Thermo Fisher Scientific	Cat# 25-9185-42; RRID: AB_2573540
mouse anti-FoxP3 V450 (Clone 259D/C7)	BD Biosciences	Cat# 561182; RRID: AB_10564088
mouse anti-CD4 BV650 (Clone L200)	BD Biosciences	Cat# 563737
mouse anti-CD8 BV786 (Clone RPA-T8)	BD Biosciences	Cat# 563823
mouse anti-CXCR3 BUV395 (Clone 1C6/CXCR3)	BD Biosciences	Cat# 565223
mouse anti-CD20 BUV737 (Clone 2H7)	BD Biosciences	Cat# 564432
mouse anti-CD95 APC (Clone DX2)	BD Biosciences	Cat# 561978; RRID: AB_10895117
mouse anti-CD3 Alexa Fluor 700 (Clone SP34.2)	BD Biosciences	Cat# 557705; RRID: AB_396814
mouse anti-CD27 APC-e780 (Clone O323)	Thermo Fisher Scientific	Cat# 47-0279-42; RRID: AB_1272040
Fixable Viability Dye Aqua	Invitrogen	Cat# L34966
Bacterial and Virus Strains
BG505.W6M.ENV.C2	Wu et al., 2006 / NIH AIDS Reagent Program	Cat# 11518
MG505.W0M.ENV.A2	Wu et al., 2006 / NIH AIDS Reagent Program	Cat# 11528
MG505.W0M.ENV.H3	Wu et al., 2006 / NIH AIDS Reagent Program	Cat# 11529
12 virus panel – global isolates	deCamp et al., 2014	N/A
Chemicals, Peptides, and Recombinant Proteins
ISCOMATRIX	CSL Behring	N/A
BG505 SOSIP.664 (HEK293T produced)	Sanders et al., 2013	N/A
BG505 SOSIP.664 (CHO produced)	Catalent	N/A
BG505 SOSIP.v4.1	de Taeye et al., 2015	N/A
BG505 SOSIP.v5.2	A.T.d.l.P. et al., unpublished data	N/A
BG505 NFL	Sharma et al., 2015	N/A
BG505 Olio6	D.W.K. et al., unpublished data	N/A
BG505 Olio6 CD4-KO	D.W.K. et al., unpublished data	N/A
BG505 V3-peptide TRPNNNTRKSIRIGPGQAFYATG	A&A Labs LLC	N/A
BG505 V3-peptide biotin TRPNNNTRKSIRIGPGQAFYATGGAGK-biotin	GenScript	N/A
TAPNNFTVKSIRIGPGAFYYMGGAGK-biotin	GenScript	N/A
His-Tag biotin peptide biotin-ALDGGGGSHHHHHHHH	A&A Labs LLC	N/A
Evans Blue dye	Sigma Aldrich	Cat# E2129
DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine	Avanti Polar Lipids	Cat# 850345
DGS-NTA(Ni): 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl] (nickel salt)	Avanti Polar Lipids	Cat# 790404
MPB: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide] (sodium salt)	Avanti Polar Lipids	Cat# 870012
Cholesterol	Sigma-Aldrich	Cat# C8667
Experimental Models: Cell Lines
TZM-bl cells	NIH AIDS Reagent Program	Cat# 8129
Experimental Models: Organisms/Strains
Indian-origin rhesus macaques (outbred)	AlphaGenesis Inc	N/A
Recombinant DNA
pSG3Δenv plasmid	NIH AIDS Reagent Program	Cat# 11051
Software and Algorithms
Prism v7.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
FlowJo v9.9.4	FlowJo	https://www.flowjo.com
Other
Mini-osmotic pumps (0.5 μL/hr, 14 day release)	ALZET	Model 2002