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. 2017 Jun 24;36:86. doi: 10.1186/s13046-017-0559-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — HOXB7 facilitated hepatoma cell proliferation by promoting stemness. a Protein level of HOXB7 in different hepatoma cell lines. b Efficiency of knockdown and overexpression HOXB7 in SMMC-7721 and HepG2 cells, respectively. c Location of HOXB7 in SMMC-7721 and HepG2 cells. d Cell Titer-Blue assay was used to examine the effect of HOXB7 on the proliferation of SMMC-7721 and HepG2 cells. HOXB7 promoted the proliferation of hepatoma cells (P < 0.05, respectively). e Analysis of clone formation in SMMC-7721 and HepG2 cells. HOXB7 promoted clone formation of hepatoma cells (P < 0.05, respectively). f Analysis of sphere formation in SMMC-7721 and HepG2 cells. HOXB7 accelerated sphere formation of hepatoma cells (P < 0.05, respectively). g, h Hepatoma stem cell markers EPCAM and NANOG expression level after HOXB7 knockdown and overexpression