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. 2017 Jun 24;10:306. doi: 10.1186/s13071-017-2250-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immune responses of Batf3 ^−/− and B6 mice after S. japonicum infection. Th1, Th2, Tc1, and Tc2 immune response were detected by FCM at 0, 3, 6 and 9 weeks post-infection of each group. Tc1 cell responses are stronger in S. japonicum infected Batf3 ^−/− mice, Th1, Th2, and Tc2 cell responses show no significant difference between Batf3 ^−/− and WT B6 mice after S. japonicum infection. a, b Percentages of CD3⁺CD4⁺IFN-γ⁺ (Th1) gated from CD3 + T cells analysed by FCM. c, d Percentages of CD3⁺CD4⁺IL-4⁺ (Th2) gated from CD3 + T cells analysed by FCM. e, f Percentages of CD3⁺CD8⁺IFN-γ⁺ (Tc1) gated from CD3 + T cells analysed by FCM. g, h Percentages of CD3⁺CD8⁺IL-4⁺ (Tc2) gated from CD3⁺T cells analysed by FCM. Results are representative of two independent experiments. Each bar represents the mean ± SEM from six mice per group. (*P < 0.05, **P < 0.01)