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. 2005 Feb 7;102(7):2496–2501. doi: 10.1073/pnas.0409849102

Fig. 1.

Fig. 1.

Design and verification of targeted alleles. (A) OGH-TG. (Upper) OGH-TG. (Lower) WT Rosa26. The arrow-labeled OGH indicates adenovirus splice acceptor, mouse OGH cDNA coding sequence, and rabbit β-globin polyA. The arrow-labeled PGK-neo indicates mouse PGK promoter, Tn5 neo, and gene mouse PGK polyA. Restriction sites and probe used for Southern blotting are shown. (B) OGH knockout/lacZ knock-in. (Upper) Endogenous mouse OGH gene. (Lower) Targeted allele. Restriction sites, predicted band size, and probe used for Southern blotting are shown. (C) Southern blot analysis of tail DNA from WT and transgenic (OGH-TG) animals confirming correct targeting. (D) TaqMan analysis of total RNA from three OGH-TG mice and three WT littermates (one male and two females, for each group). H, heart; K, kidney; L, liver. (E) Southern blot analysis of tail DNA from WT, heterozygous, (OGH+/-), and homozygous knockout (OGH-/-) animals.