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. 2005 Feb 8;102(8):2892–2897. doi: 10.1073/pnas.0409781102

Fig. 4.

Fig. 4.

EMSA demonstrates the activation of NF-κB in RASF after stimulation with microparticles. Lane 1, free probe without nuclear extracts; lane 2, nuclear extract from unstimulated RASF, with NF-κB probe; lane 3, nuclear extract from unstimulated RASF, with NF-κB probe and cold NF-κB probe; lane 4, nuclear extract from RASF stimulated with microparticles for 10 min, with NF-κB probe; lane 5, nuclear extract from RASF stimulated with microparticles for 10 min, with NF-κB probe and cold NF-κB probe; lane 6, nuclear extract from RASF stimulated with microparticles for 60 min, with NF-κB probe; lane 7, nuclear extract from RASF stimulated with microparticles for 60 min, with NF-κB probe and cold NF-κB probe. Signals for NF-κB–DNA complexes in lane 4 and especially lane 6 confirm an activation of the transcription factor NF-κB. No activation of NF-κB signaling was observed in nonstimulated, serum-starved fibroblasts (lanes 2 and 3). The signal of the NF-κB–DNA complexes disappeared completely after adding an excess of unlabeled cold NF-κB probe (lanes 5 and 7).