Fig. 2.

The influence of type I IFN signaling on antibody-mediated demyelination. a The left panel shows a bar graph with activation of astrocytes and microglia in and around demyelinated areas in animals injected with anti-MOG + C or anti-MOG alone. The right panel shows representative images of GFAP and Iba-1 staining from mice injected with anti-MOG + C. Results in the bar graph are shown as mean ± SEM of semi-quantitative scores (arbitrary units), the total number of animals in each group is shown underneath columns. Original magnification in the representative images is ×20. b Bar graphs showing IFN-β and CXCL10 gene expression in animals injected with anti-MOG + C, PBS, or anti-MOG alone. Data are shown as mean ± SEM, the total number of animals in each group is shown underneath columns. The data represent pooled results from at least three separate experiments. IFN-β gene expression was not detected (ND) in three samples from each group. c Anti-MOG + C was injected into CC of either C57BL/6 or IFNAR1-deficient mice, and pathology was assessed 2 days later. Bar graph shows percentage demyelination. Data were analyzed using two-tailed Mann–Whitney U test. Results are shown as mean + SEM, the total number of animals in each group is shown underneath columns. The data represent pooled results from three separate experiments