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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Biol Rhythms. 2016 Dec 20;32(1):35–47. doi: 10.1177/0748730416680904

Figure 2. Automated clustering reveals that, following removal of TTX, the dorsal region attained a stable period after the ventral region and an earlier time of peak PER2 expression.

Figure 2

A) Clustering of cells in the left half of the representative SCN from Figure 1 based on the phasing of their PER2 expression (colors as in Fig. 1C). B) VIP immunolabeling (green) localized predominantly within the red group of cells identified by their PER2-based clustering. C) AVP immunolabeling (cyan) localized within both clusters of cells. D) The mean PER2 expression following removal of TTX within the ventral (red) and dorsal (blue) groups of cells. Note that PER2 expression within the dorsal cells gradually increased in amplitude and synchronized to peak just prior to the daily peaks in the ventral cells. E) The mean periods of cells in the ventral (red) cluster reached their stable period sooner than those in the dorsal SCN (blue). F) The peak times (mean ± SD) of daily PER2 expression gradually synchronized within the SCN so that, in steady state, dorsal (blue) cells peaked 2.4 h earlier than ventral cells (red).