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. 2005 Jan 26;33(2):525–535. doi: 10.1093/nar/gki178

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Size-dependent HPLC of poly(dG)–poly(dC) at high-pH. Poly(dG)–poly(dC) synthesized with Klenow exo⁻ as described in Figure 1 (solid curve) and poly(dG)–poly(dC) from Sigma (dashed curve) were pretreated for 15 min at room temperature in 0.1 M KOH. A total of 100 μl of each DNA sample were applied onto TSKgel G-DNA-PW column (7.8 × 300 mm) and eluted at room temperature with 0.1 M KOH at a flow rate of 0.5 ml/min. The length of the synthesized poly(dG)–poly(dC) estimated by gel electrophoresis as shown in Figure 1 is equal to 7 kb. Elution was followed at 260 nm. Insets present normalized absorbance spectra obtained using diode-array detection of fractions eluted at the time points indicated by the arrows.