Table 2.

Priming of poly(dG)–poly(dC) synthesis with various double-stranded synthetic DNA sequences

Oligonucleotide	Priming of poly(dG)–poly(dC) synthesis
5′-GGGGGGGGGGGGA-3′–5′-CCCCCCCCCCCCA-3′	No
5′-AGGGGGGGGGGGG-3′–5′ACCCCCCCCCCCC-3′	Yes
5′-GGGGGGGGGGGGA-3′–5′-CCCCCCCCCCCCC-3′	No
5′-GGGGGGGGGGGGG-3′–5′-ACCCCCCCCCCCC-3′	Yes
5′-GGGGGGGGGGGGG-3′–5′-CCCCCCCCCCCCA-3′	No
5′-AGGGGGGGGGGGG-3′–5′-CCCCCCCCCCCCC-3′	Yes
5′-Flu-GGGGGGGGGGGG-3′–5′-TAMRACCCCCCCCCCCCC-3′	Yes
5′-NH2-GGGGGGGGGGGG-3′–5′-NH2-CCCCCCCCCCCCC-3′	Yes
5′-GGGGGGGGGGGG-NH2-3′–5′-CCCCCCCCCCCCC-NH2-3′	No
5′-SH-GGGGGGGGGGGG-3′–5′-SH-CCCCCCCCCCCCC-3′	Yes

Reactions were performed as described in Materials and Methods with 5 μM of template-primers and 10 μg/ml of Klenow exo⁻. The products of synthesis were analyzed by electrophoresis as in Figure 3.