Table 3.

Extension rates for the different template-primer sequences by Klenow exo⁻

Template-primer	Product of extension	TN (min−1)
5′-GGGGGGGGGG3′–5′CCCCCCCCCC-3′	5′-GGGGGGGGGGG-3′–5′-CCCCCCCCCCC-3′	60
5′-GGTGGGGGGGGGA-3′–5′-CCCCCCCCCCCCA-3′	5′-GGTGGGGGGGGGA-3′–5′-CCCCCCCCCCCCACC-3′	50
5′-GGGTGGGGGGGGA-3′–5′-CCCCCCCCCCCCA-3′	5′-GGGTGGGGGGGGA-3′–5′-CCCCCCCCCCCCACCC-3′	20
5′-GGGGTGGGGGGGA-3′–5′-CCCCCCCCCCCCA-3′	5′-GGGGTGGGGGGGA-3′–5′-CCCCCCCCCCCCACCCC-3′	5.6
5′-GGGGGTGGGGGGA-3′–5′-CCCCCCCCCCCCA-3′	5′-GGGGGTGGGGGGA-3′–5′-CCCCCCCCCCCCACCCCC-3′	0.3

The reactions were performed and the rates were determined as described in the legend to Figure 8. The rates are expressed in a number of the enzyme's turnovers per minute.