Table 1.

The results of the fitting procedure for LED quadratic and linear fits and for Sph8 and TF6 bead fits. The upper part shows flowQB output results for c0, c1 and c2 and their standard errors on 8 channels of one LSR-II instrument (LSR-IIF, later run). The c2 P-value is included for the LED-quadratic fit. The lower section shows the ratio of standard errors to fit parameter values as percent (%). The “Bead CV₀ Estimates” are the square roots of the corresponding c2 values expressed as percent (%). The “LED linear fit results expressed in terms of Spe units” section shows LED linear results expresses as B_Spe and Q_I. The “ Q_I estimates for other methods relative to the LED linear Q_I” section at the bottom shows the similarity of the different estimators of the Spe scale.

LSR-IIF, later run Primary Fit Output		Dye	APC	APC-Cy7	FITC	PE	PE-Cy7	PerCP-Cy55	V450	V500-C
	LED quadratic	c0	129	1047	284	164	152	284	1437	343
		c0 StdErr	0.9	5.5	1.4	1.1	0.8	3.0	9.8	9.0
		c1	4.09	11.23	1.09	1.21	3.03	5.35	1.59	2.67
		c1 StdErr	0.0171	0.0659	0.0064	0.0074	0.0205	0.0459	0.0194	0.0679
		c2	9.60E-7	8.19E-7	7.08E-7	10.1E-7	8.14E-7	1.21E-7	10.9E-7	17.8E-7
		c2 StdErr	8.56E-7	41.17E-7	1.71E-7	2.60E-7	43.3E-7	22.7E-7	7.62E-7	24.4E-7
		c2 P value	0.284	0.847	0.001	0.002	0.854	0.958	0.179	0.481
	LED linear	c0	129	1047	285	165	152	284	1441	344
		c0 StdErr	0.9	5.2	2.0	1.5	0.7	2.8	10.0	8.7
		c1	4.10	11.22	1.07	1.20	3.02	5.35	1.56	2.64
		c1 StdErr	0.0143	0.0501	0.0072	0.0084	0.0150	0.0359	0.0134	0.0525
	Sph8	c0	84	729	180	156	119	153	1055	237
		c0 StdErr	67.2	176.1	6.1	4.7	5.3	8.8	291.0	33.8
		c1	4.28	12.47	1.15	1.31	3.11	5.46	2.21	2.79
		c1 StdErr	0.4519	1.5455	0.0398	0.0300	0.0805	0.1268	0.4043	0.1359
		c2	42.062E-5	22.465E-5	22.862E-5	17.7E-5	19.714E-5	22.06E-5	7.20E-5	5.52E-5
		c2 StdErr	50.8E-6	91.0E-6	8.39E-6	4.38E-6	12.8E-6	12.0E-6	17.5E-6	7.16E-6
	TF6	c0	70	913	278	193	131	187	1361	293
		c0 StdErr	41.3	28.9	9.0	7.9	4.2	8.8	238.3	34.9
		c1	4.65	11.10	1.25	1.42	2.97	5.29	1.91	2.78
		c1 StdErr	0.2988	0.2386	0.0386	0.0565	0.0850	0.1619	0.5809	0.3017
		c2	8.81E-5	17.696E-5	8.48E-5	16.875E-5	25.717E-5	18.084E-5	50.003E-5	59.019E-5
		c2 StdErr	16.6E-6	12.3E-6	2.93E-6	6.41E-6	18.0E-6	11.5E-6	68.9E-6	70.1E-6
RelStdErr for the fit parameters			APC	APC-Cy7	FITC	PE	PE-Cy7 P	PerCP-Cy55	V450	V500-C
	LED quadratic	c0 Relative StdErr (%)	0.66	0.52	0.49	0.65	0.51	1.05	0.68	2.62
	LED linear	c0 Relative StdErr (%)	0.66	0.50	0.71	0.91	0.48	1.00	0.69	2.53
	Sph8	c0 Relative StdErr (%)	80.04	24.15	3.38	2.99	4.45	5.79	27.57	14.24
	TF6	c0 Relative StdErr (%)	59.01	3.17	3.25	4.11	3.22	4.68	17.51	11.91
	LED quadratic	c1 Relative StdErr (%)	0.42	0.59	0.59	0.61	0.68	0.86	1.23	2.55
	LED linear	c1 Relative StdErr (%)	0.35	0.45	0.67	0.70	0.50	0.67	0.86	1.99
	Sph8	c1 Relative StdErr (%)	10.55	12.39	3.45	2.28	2.59	2.32	18.28	4.86
	TF6	c1 Relative StdErr (%)	6.43	2.15	3.09	3.98	2.86	3.06	30.38	10.84
	LED quadratic	c2 Relative StdErr (%)	89.20	−509.22	−24.22	−25.68	−532.18	−1876.62	−69.69	−137.56
	LED linear	c2 Relative StdErr (%)
	Sph8	c2 Relative StdErr (%)	12.07	40.51	3.67	2.48	6.49	5.45	24.27	12.98
	TF6	c2 Relative StdErr (%)	18.84	6.95	3.46	3.80	7.00	6.36	13.78	11.88
Bead CVo Estimates			APC	APC-Cy7	FITC	PE	PE-Cy7	PerCP-Cy55	V450	V500-C
	Sph8	c2 CVo (%)=100*sqrt(c2)	2.05	1.50	1.51	1.33	1.40	1.49	0.85	0.74
	TF6	c2 CVo (%)=100*sqrt(c2)	0.94	1.33	0.92	1.30	1.60	1.34	2.24	2.43
LED linear fit results expressed in terms of Spe units
	BSpe for LED lin	(=c0/c12)	8	8	247	115	17	10	588	49
	QI for LED Iin	(=1/c, or Spe/Signal Unit)	0.24	0.09	0.93	0.84	0.33	0.19	0.64	0.38
QI estimates for other methods relative to the LED linear QI
Other QI/LED [in QI(=LED Iin c1/Other c1)		LED quadratic	1.00	1.00	0.98	0.99	1.00	1.00	0.99	0.99
		Sph8 AE01	0.96	0.90	0.93	0.91	0.97	0.98	0.71	0.94
		TF6 FC3MV	0.88	1.01	0.86	0.84	1.02	1.01	0.82	0.95