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. 2017 Jun 26;8:669. doi: 10.3389/fimmu.2017.00669

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Copyright © 2017 Li, Wang, Hao, Han, Xia, Li, Chen, Tanaka and Qiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Th17-related cytokines and the percentage of Th17 cells could be affected by the presence or not of CD169⁺ subset in mesenteric lymph nodes (mLNs). WT and CD169-diphtheria toxin receptor (DTR) mice with diphtheria toxin treatment were both orally treated with 3.5% dextran sulfate sodium (DSS) for 7 days. (A) Th17-related cytokines expression levels in mLNs. (B) Real-time PCR analysis of chemokines in mLNs of DSS-treated WT mice and CD169-DTR mice. (C) Flow cytometry analysis of Th17 cells in mLNs obtained from WT control mice, WT colitis mice and CD169-DTR colitis mice and statistical analysis of the result of FACS. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control. Data are representative of two independent experiments.