Figure 3.

Effect of UL42 on the steady-state rates of ATP hydrolysis at limiting UL9 concentration. A limiting concentration of UL9 (25 nM) was incubated to equilibrium with a fixed concentration (50 nM) of 38mer or 23/38mer DNA (A and B, respectively). Reactions were initiated by the addition of MgCl₂ alone (filled circles) or with MgCl₂ and UL42 to achieve a 50 nM final concentration (filled squares) and hydrolysis of ATP was monitored over a 90 min period at 37°C.